- Earth Temple - Eye of Cthulhu
- Corruption Temple - Eater of Worlds
- Obsidian's Volcano - Slogra and Gaibon (optional)
- The Orid's Jungle Ruins - Skeletron
- The Forgotten City - The Ancient Jungle Wyvern
- Fire Temple - The Wall of Flesh
- Hallowed Caverns - The Rage
- The Wyvern Mage's Mountain Fortress - Wyvern Mage (optional)
- The Frozen Ocean - The Sorrow
- Elengad's Desert Ruins - The Hunter
- The Twin Peaks of Arazium - The Twins
- Shadow Temple - The Destroyer
- Temple of the Machine/Water Temple - Skeletron Prime
- Attraidies' Sky Temple - Attraidies aka the Mindflayer King
Unconnected Optionial Boss Fights:
Attraidies' Sky Temple - Death
Corruption Temple - Marilith
Beneath the Western Ocean - Lich King
Shadow Temple - Kraken